Theoretical and practical methodology for the use of the polymerase chain reaction (PCR): Strategies for optimizing long-PCR performance including amplification of long fragments of DNA.

• BIOSCI 37 Course Page

Introduction to good laboratory practices in genomics: EPA and FDA regulatory protocols and standard operating procedures for designing experiments; recording observations, analyzing and reporting results; safety, instrumentation, and equipment maintenance; and documenting and presenting project/experiment results.

• BIOSCI 32 Course Page

Proficiency in using MacVector software: Mining GenBank and preparation of GenBank files, clustal alignments, gene annotation, and frame orientation of protein-coding genes.

• BIOSCI 61 Course Page

Introduction to genetics and genomic technologies: Principles of genetics and data collection (DNA and RNA structure, mitochondrial DNA, nucleic acid replication, protein synthesis, mitosis-meiosis, Mendelian principles, mutation, molecular character alignment, evolution and phylogenetic reconstruction), combined with data handling (bioinformatics mining using GenBank data), and scientific writing; includes guest speakers and on-site tours of genomics institutions and labs.

Shotgun sequencing and techniques using Covaris and the Hydroshear in DNA preparation.

Proficiency in using MacVector software: Mining GenBank and preparation of GenBank files, clustal alignments, gene annotation, and frame orientation of protein-coding genes.

• BIOSCI 61 Course Page

Introduction to PAUP software: Character vs. character states, parsimony, distance and the methods, shared ancestry vs. homoplasy (reversals vs. parallelisms).