Sequence Analysis Using MacVector
BIOSC 61: 1 unit, 1 hour lecture
Section I: 31192 Lec 09:00-12:20, 01:00-05:50 SSU
07/13/2013 - 07/14/2013 Meets at 860 Atlantic Ave., Alameda
Recommended preparation: CIS 1
Prerequisites: BIOSC 38, 40, AND 41*
* The prereqs are not yet available. Please contact us for add codes if you are interested in the course.
Download [PDF] this form for Bob to sign to skip the prereqs. Proficiency in using MacVector software: Mining GenBank and preparation of GenBank files, clustal alignments, gene annotation, and frame orientation of protein-coding genes.
Class resources:
Sequence analysis
Using analytical methods to understand the function, structure and evolution of DNA, DNA or amino acid sequences. Using software we can work with our DNA to align and assemble it, an analyze two genomes to determine relation (are they homologous?), look for point mutations and single nucleotide polymorphims (SNPs). Looking at single genomes we can determine active sites, sequence motifs, reading frames, introns, exons, post translational modification sites and regulatory elements. MacVector
Sequence editing, multiple sequence alignment, reading frame analysis, phylogenetic reconstruction, database and BLAST searching, protein analysis, contig assembly, chromatogram editing, cDNA alignment, dot plots, coding region analysis, restriction anaylsis and PCR primer design. Allows plugins for phred and phrap. PAUP
Computational phylogenetics software to infer evolutionary trees of organisms based on their DNA. Command line-only on Mac OS X 10.5 and later. Phred
Used to identify a base sequence from fluorescence data obtained obtained from electrophoretic sequencing. This generates a 'quality' score used in Phrap. Phrap
Used for sequence assembly. MacVector allows a graphical interface for using Phrap. Consed
Used to view or edit DNA sequence assemblies created in Phrap. MacClade |
|
Merritt Microscopy | Docs © 2012 Profile Genomics | |